Helicon Focus, is an excellent tool that creates a complete focused image from several partially focused images.
    This amazing tool is specially made to make optic microscope image processing tasks simpler. This application has the capability to deal with the depth-of-field problem and is applicable for real time microphotography and hyper-focal landscape photography. The tool has the capability to create a perfectly focused image from several partially focused images. The key features supporting this tool are: auto adjust and resize images, ability to process multiple images at once, Internal workflow always uses 16 bit color depth to preserve colors, applies no limitations on image resolutions, supports dust map, automatic brightness adjustment of adjacent images, can read; RAW, 8bit and 16bit TIFFs, JPEG, JPEG 2000, BMP and so on.
    Helicon Focus, the highly focused tool with which you can be assured to get high quality output and perfectly focused images.